Stromal layers are consistently generated from bone marrow cells from humans, rats and mice.

 

Stromal layers can also be generated from cells from adipose tissues obtained either by liposuction or abdominal dermolipectomy and from muscle connective tissue treated by enzymes (collagenase, dispase) that release the culture-initiating cells. The efficiency in generating a stromal layer from blood cells (circulating peripheral or placental) is comparatively lower, possibly as a result of different and as yet undefined culture requirements, or as a reflection of the need to mobilize circulating stromal progenitors from their niches using specific, and as yet undefined, procedures.

Finally, it is expected that cells from the vascular network of different tissues would initiate stromal layers, and this has indeed ben reported recently for sub-endothelial cells from the umbilical cord.

 

One of the objectives of this project is to define the best procedure to collect autologous MSCs from stromal layers from the different sources. It is conceivable that stromal cells from one source may be more proliferative or more appropriate for a specific lineage and this will be tested. By the end of this study we should be able to provide protocols for isolation of i) undifferentiated or ii) committed MSCs, iii) fully-differentiated progeny or iv) progeny blocked at a certain differentiation stage. A milestone will be the isolation of cells from the different categories that will be frozen and available to other (4-8) workblocks for further in vitro studies (organization into full-fledged tissue) and transplantation in the different animal models.

 

 This workblock is managed by P Charbord working with:

 

 

 

 

       A Hatzfeld                              P Bianco                                      V Paunescu                                 R Oostendorp

       (CNRS -France)                 (Uni Roma -Italy)                    (Uni Timisoara -Romania)               TUM (Germany)

 

 

                            

 

 

 

 

    

    

H Papadaki                           L Dimitriou                                        J P Mouscadet                               N Rougier

(Uni Crete -Greece)               (Uni Crete -Greece)                     (Abcys—France)                      (Biopredic-France)

 

            

 

 

 

 

 

                     

      L Casteilla                            J T Vilquin

    (CNRS -France)                  (Inserm - France)

 

                 

Work block 1 : Isolation of Mesenchymal Stem Cells